SIELC - Spektrotek

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SIELC, eski Pfizer bilim insanlarının kurduğu bir Amerikan teknoloji şirketidir. Özellikle Kolon Kromatografisi alanında “Mixed-mode” ayırma teknolojilerinde özelleşmiştir. Üretmiş olduğu Kromatografi kolonlarının pek çok zorlu analizde getirdiği kolaylık ve çözümlerle rakiplerinden ayrılmaktadır.

Üretmiş olduğu çok özel kolonlardan bazıları aşağıda listelenmiştir. Burada listelenenler ve daha fazlası için Spektrotek uzmanlığına başvurabilirsiniz: info@spektrotek.com

SIELC kolonları: PrimeSep, NewCrom, Obelisc, CannSep, Legacy, Promix, SHARC, Solid-Core, Speciality Columns

Aşağıda seçilmiş bazı kolonlar, özellikleri ve kullanıldıkları Aplikasyon / Uygulamaları bulabilirsiniz.

Primesep

Primesep 100 is a reverse-phase analytical column with embedded acidic ion-pairing groups

This is the most versatile column of the entire Primesep family for separation of a broad range of compounds.

Improves retention of basic compounds by cation-exchange mechanism.
Separates acids by ion-exclusion mechanism.
Retains neutral compounds by reverse-phase mechanism.
All mobile phases are LC-MS and preparative chromatography compatible.

Primesep 100 columns are available in all standard dimensions

Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 µm, 5 µm, 3 µm, 2.7 µm (solid core)
Pores: 100 A

Primesep 100 Application Examples

HPLC Separation of β‑Hydroxy-β-methylbutyric Acid And Leucine

HPLC Determination of Ritodrine

HPLC Determination of Folic Acid

HPLC Method for Analysis of Gabapentin Tablets

HPLC Method for Analysis of Thymol Blue

Separation of allo-D-isoleucine and L- Isoleucine on Primesep 100 HPLC column

2,4-D Retention on Primesep 100 and Obelisc R

1-Naphthaleneacetic acid (NAA) Separation on Primesep and Obelisc Mixed-Mode Columns

16a-Hydroxyestradiol Separation on Mixed-Mode HPLC Columns

4-CPA Analysis on Obelisc and Primesep Mixed-Mode Columns

Amino Acids Analysis in Acid Gradient Condition

Amino Sugars Analysis in Ion Exchange and Reverse Mode

Analysis of Folic Acid and Related Impurities

Analysis of Nicotinic Acid in Nicotinamide

Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds

Controlling Retention of 2,4-DB on Mixed-Mode HPLC Columns

Controlling Retention of Azinphos-ethyl on Primesep and Obelisc HPLC Columns

Dewetting Study in Aqueous Conditions

Dichlorprop (2,4-DP) Analysis on Primesep and Obelisc HPLC Columns

Diclobutrazol Separation on Primesep and Obelisc HPLC Columns

Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds

Effect of Buffer Concentration on Resolution of Neutral Compounds (Prednisolone and D-Glucose Pentaacetate)

Effect of Concentration of Organic Modifier on Retention of Cation and Anions

Folpet Analysis on Primesep 100 and Obelisc R HPLC Columns

HPLC Analysis of 17a-Ethynylestradiol

HPLC Analysis of 2,4,5-T on Mixed-Mode Columns

HPLC Analysis of 5-Fluorouracil on Primesep Columns with LC/MS Compatible Mobile Phase

HPLC Analysis of Active Drug and Amino Acids in a Formulation

HPLC Analysis of Bentazon on Primesep and Obelisc Mixed-Mode Columns

HPLC Analysis of Calcium Ions in Maple Syrup

HPLC Analysis of Cheese Extract using Mixed-Mode Chromatography

HPLC Analysis of Citrulline and Arginine on Primesep Columns

HPLC Analysis of Dichloran on Mixed-Mode Columns

HPLC Analysis of Diphenamid on Primesep 100 and Obelisc R

HPLC Analysis of Fatty Acids with MS Compatible Conditions

HPLC Analysis of Fentin on Obelisc and Primesep Mixed-Mode Columns

HPLC Analysis of Inosine on Primesep 100 Column

HPLC Analysis of MCPA on Primesep and Obelisc Mixed-Mode Columns

HPLC Analysis of Motrin Cough & Cold Composition

HPLC Analysis of Piperazine on Primesep Columns

HPLC Application for Analysis of Sodium Succinate on Primesep 100

HPLC Application for Retention of Guanidine by Mixed-Mode Chromatography

HPLC Application for Simultaneous Separation of Amino Acids, Hydrophilic Acidic and Hydrophobic Neutral Compounds

HPLC Diazinon Analysis on Primesep 100 and Obelisc R columns

HPLC Method for Analysis of Fluconazole

HPLC Method for Determination of Lysine in Ibuprofen Lysine Composition

HPLC Retention Control of Antibiotics in Toothpaste

HPLC Retention of Aminopyrazole

HPLC Retention of Chlorpyrifos-methyl on Primesep 100 and Obelisc R Mixed-Mode Columns

HPLC Retention of Copper Ion on Primesep 100 Mixed-Mode Cation-Exchange Columns

HPLC Retention of Glucosamine

HPLC Retention of Pirimicarb on Primesep 100 and Obelisc R

HPLC Separation of 2-Naphthoxyacetic acid

HPLC Separation of 8 Generic Compounds on Primesep Columns

HPLC Separation of a Complex of Quinolone Antibiotics

HPLC Separation of Acidic, Basic and Zwitterionic Compounds on Special Columns for Retention of Polar Compounds

HPLC Separation of Albuterol and Amoxicillin

HPLC Separation of Amino Acids

HPLC Separation of Amino Acids in Supplements Capsules

HPLC Separation of Amino Acids in Supplements Composition in Mixed-Mode

HPLC Separation of Aminopyridines in Non-Aqueous Mobile Phase

HPLC Separation of Aromatic Sulfonamides and Hydrozine

HPLC Separation of Benzenesulfonamide and Sulfanilamide

HPLC Separation of Caffeine and Phenylephrine

HPLC Separation of Captan on Obelisc R and Primesep 100 Columns

HPLC Separation of Carbofuran on Obelisc R and Primesep 100 Columns

HPLC Separation of Carboxylic Acids

HPLC Separation of Catecholamines on Primesep 100 Column with Phosphate Buffers

HPLC Separation of Cidofovirs Mixture

HPLC Separation of Codeine, Oxycodone, and Hydrocodone

HPLC Separation of Components of Excedrin

HPLC Separation of DMSO and Glycine by Mixed-Mode Chromatography

HPLC Separation of DOPAC, Homovanillic Acid and Dopamine on the Primesep 100 Column

HPLC Separation of Epinephrine and Epinephrine Sulfonate

HPLC Separation of Estrone

HPLC Separation of Ethanolamines

HPLC Separation of GABA and GLU

HPLC Separation of Glutamic Acid and GABA

HPLC Separation of Glyphosate Production Intermediates

HPLC Separation of Guaifenesin and Terbutaline

HPLC Separation of Guanidine and Benzoic Acids on Primesep 100 Column

HPLC Separation of Hydrocortisone and Hydrocortisone’s Degradation Products

HPLC Separation of Impurities of Aminosugar

HPLC Separation of Isomers of Amino Salicylic Acid

HPLC Separation of Isomers of Aminobenzoic Acid

HPLC Separation of Metribuzin on Mixed-Mode HPLC Columns

HPLC Separation of Nabam on Mixed-Mode Columns

HPLC Separation of Phenylalanine, Glucosamine, and Tryptophan on Mixed-Mode Column

HPLC Separation of Phenylephrine with Tannic Acid Formulation

HPLC Separation of Phosphamidon on Mixed-Mode Columns

HPLC Separation of Phthalic Acids using Hydrogen Bonding

HPLC Separation of Pyridinecarboxylic Acids

HPLC Separation of Serine, a-Methylserine, GABA, and Methionine Mixture

HPLC Separation of Toluidine Isomers

HPLC Separation of Triclopyr

HPLC Separation of TRIS

Hydrophobic and Hydrophilic Compound Separation

Loading Study for Preparative Chromatography

MCPB Analysis Using Obelisc and Primesep Analytical HPLC Columns

Mecoprop Analysis on Primesep and Obelisc Mixed-Mode HPLC Columns

Nickel Contamination in Hydrogenation Products

Potassium Analysis in Organic Reaction Product

Retaining the Fungicide Carboxin on Primesep and Obelisc HPLC Columns

Retention Control of Bromacil on Primesep and Obelisc Mixed-Mode Columns

Retention of Aminolevulinic Acid in Mixed-Mode System

Retention of Monocrotophos on Primesep 100 and Obelisc R Columns

Retention of the Herbicide Dicamba on Mixed-Mode HPLC Columns

Reverse Elution Order in Separation of Alkali Cation

Reverse Mode vs Normal Mode: Separation of Aromatic Compounds

Separating Triadimefon from Impurities on Primesep and Obelisc HPLC Columns

Separation of Diacid: Ion Exclusion mode

Separation of Glyphosate by Cation Exchange and Anion Exchange

Separation of Guanidine

Separation of Isomers of Aminobenzoic Acid

Separation of Metal Ions on Primesep 100 Column

Separation of Oxytetracycline and Chlortetracycline in Mixed-Mode

Separation of Sodium, Potassium, Lithium, and Ammonium in Mixed-Mode HPLC

Separation of Steroids in Reverse and Normal Mode on Primesep 100 Column

Separation of Sulfonamides and Phenylhydrazine by Mixed-Mode HPLC

Separation of Sulphonium Ions in Polar Organic Mode

Separation of Thiabendazole on Mixed-Mode HPLC Columns

Separation of Zn and Mn on Primesep 100 Column

Simultaneous Separation of Cations and Anions

Terbufos Analysis on Primesep 100 and Obelisc R Columns

Zn and Na Quantitation in Drug Substances

Primesep A is a reverse-phase analytical column with embedded strong acidic ion-pairing groups.

Primesep A - Acidic

Improves retention of weak basic compounds by cation-exchange mechanism
Separates acids by ion-exclusion mechanism
Retains neutral compounds by reverse-phase mechanism
All mobile phases are LC-MS and preparative chromatography compatible

Primesep A columns are available in all standard dimensions

Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

HPLC Retention of Aminopyrazole

HPLC Separation of Taurine

Mixed-Mode Retention and Separation of Ammonia, Primary and Secondary Amines on Primesep A Column

Neutral, Base, and Acid on Primesep A Column

Separation of Simple Amines and Ammonia

TFA Concentration Effect on Retention of Tricine

Primesep AB is a reverse-phase column with embedded acidic and basic ion-pairing groups.

Improves retention of strong acidic compounds and strong basic compounds by ion-exchange mechanism
Retains neutral compounds by reverse-phase mechanism
All mobile phases are LC-MS and preparative chromatography compatible

Primesep AB columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep AB Application Examples

HPLC Analysis of Epinephrine and Epinephrine Sulfonate on Trimodal Column

HPLC Analysis of Polar Basic and Acetic Compounds on Primesep AB Column

HPLC Separation of Mixture of Paraquat and Diquat

Primesep B is a reverse-phase analytical column with embedded basic ion-pairing groups specifically designed for direct plasma analysis.

It contains a C12 carbon chain and basic groups with a pKa of 10.

Primesep B - Basic

Does not retain high-molecular weight plasma components
Retains hydrophobic and acidic components
All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 4)

Primesep B columns are available in all standard dimensions

Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 µm, 5 µm
Pores: 100 A

Primesep B Application Examples

HPLC Determination of β‑Hydroxy-β-methylbutyric Acid

HPLC Separation of Acrylic Acid and 4- Methoxyphenol on Primesep B Column

HPLC Determination of Acrylic Acid on Primesep B Column

HPLC Method for Analysis of Meclizine

HPLC Determination of Cyclopropanecarboxylic Acid

Primesep B2 is a reverse-phase analytical column with embedded basic ion-pairing groups.

Primesep B - Basic

Improves retention of acidic compounds by anion-exchange mechanism
Separates bases by ion-exclusion mechanism
Retains neutral compounds by reverse-phase mechanism
All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 5.5)

Primesep B2 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primsep B2 Application Examples

HPLC Method for Analysis of Selenious Acid

Alachlor Separation on Obelisc and Primesep HPLC Columns

Analysis of 2,4,5-Trichlorophenoxyacetic acid on Primesep and Obelisc HPLC Columns

Analysis of Naled on Primesep and Obelisc Mixed-Mode HPLC Columns

Azinphos-ethyl Analysis on Mixed-Mode HPLC Columns

Captan Separation on Obelisc and Primesep Mixed-Mode HPLC Columns

Carbofuran Separation on Primesep and Obelisc Mixed-Mode HPLC Columns

Chlorpyrifos-methyl Separation Using Obelisc and Primesep Columns

Dicamba Analysis on Primesep and Obelisc Mixed-Mode HPLC Columns

Dichlorvos Separation on Obelisc and Primesep Mixed-Mode Columns

Diphanamid Separation on Primesep and Obelisc Mixed-Mode HPLC Columns

Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds

HPLC Analysis of Cranberry Pomace Extract

HPLC Analysis of Gemfibrozil and Ibuprofen Using Mixed-Mode Chromatography

HPLC Analysis of Sulfate and Phosphate Ions on Primesep Columns

HPLC Analysis of Valproic Acid using Mixed-Mode Chromatography

HPLC Analysis of Zearalenone and Ochratoxin A on Primesep D Mixed-Mode Column

HPLC Retention of Diazacon on Primesep B2 Mixed-mode Anion-Exchange Column

HPLC Retention of Fentin Acetate

HPLC Separation of Adenosine Mono-, Di- and Triphosphate in Reversed-Phase Mixed-Mode with LC/MS Compatible Conditions

HPLC Separation of Carboxylic Acids

HPLC Separation of Chlorate and Bromate Ions

HPLC Separation of Diazinon on Obelisc and Primesep Mixed-Mode Columns

HPLC Separation of Folpet on Obelisc and Primesep Mixed-Mode Columns

HPLC Separation of Glyphosate Production Intermediates

HPLC Separation of Lactic Acid and Its Oligomers

HPLC Separation of m-Cresol Purple and Related Impurities

HPLC Separation of Nitroprusside and its Metabolites

HPLC Separation of Parabens and Benzoic Acid

HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC

HPLC Separation of Triadimefon on Obelisc and Primesep Columns

HPLC Separation of Two Vitamins: Different Polarity – Isocratic Methods

Improved Peak Shape of a Basic Compound on Primesep B2 Column

Malathion Analysis on Obelisc and Primesep Mixed-Mode HPLC Column

Monocrotophos Separation on Obelisc and Primesep Mixed-Mode HPLC Columns

Nabam Analysis on Mixed-Mode HPLC Columns

Phosphamidon Analysis Using Obelisc and Primesep Mixed-Mode HPLC Columns

Pirimicarb Analysis on Obelisc and Primesep Mixed-Mode HPLC Columns

Separation and Retention of Dextromethorphan Bromide in Mixed-Mode HPLC: Buffer Concentration Effect

Separation and Retention of Vitamins in Mixed-Mode HPLC

Separation of Maleic and Succinic Acid on Primesep B2 column

Separation Study of Fentin Acetate in Mixed-Mode HPLC

The organic acids glucuronic acid, methanesulfonic acid, and butanesulfonic acid were separated using Primesep B2 mixed-mode column. Primesep B2 is a reverse-phase column with embedded basic ion-pairing groups.

Primesep C is a reverse-phase analytical column with embedded complex-forming groups.

Primesep C - Complex

Improves retention of positively charged compounds by forming a host-guest complex
Retains neutral compounds by reverse-phase mechanism
All mobile phases are LC-MS and preparative chromatography compatible

Primesep C columns are available in all standard dimensions

Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep C Application Examples

HPLC Separation Of Mixture of Pyridylacetic Acids

Analysis of Codeine and Related Impurities in Drug Composition

Analysis of Codeine-Based Drug Composition. Effect of Buffer Concentration and Buffer pH

Analysis of Dextromethorphan-Based Composition

Analysis of Dextromethorphan-Based Drug composition. Effect on buffer pH

Analysis of Methylene Blue Purity in Reversed-Phase Cation-Exchange and Reversed-Phase Cation-Exclusion Modes

Analysis off Codeine-based Drug Composition. Effect of Buffer Concentration and Buffer PH

Bufferless Ion Separation (BLIS™) Chromatography of Aspartame: Organic Modifier Effect

Complex of Cations on Primesep C is Effected by Organic Modifier

Control of Cation Exchange Properties by pH of Primesep C Column

Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds

Effect of pH on Retention of Basic Compounds on Primesep C Columns

HPLC Analysis of Alginic Acid in Heartburn Tablets

HPLC Retention of Fentin Acetate

HPLC Retention of Obidoxime

HPLC Retention of Trimipramine

HPLC Separation of Acetaminophen, Phenylephrine, and Diphenhydramine

HPLC Separation of Acetylcholines

HPLC Separation of Antibiotics in Fish Production

HPLC Separation of Common Antibiotics in Fish Farming

HPLC Separation of Drugs

HPLC Separation of Explosives

HPLC Separation of Histamine and Phenethylamine

HPLC Separation of Isomers of Aminobutyric Acids

HPLC Separation of Lysine and Arginine from Other Amino Acids

HPLC Separation of NyQuil Active Ingredients

HPLC Separation of Polar Drugs with MS-compatible Method

HPLC Separation of Polyamines

HPLC Separation of Surfynol 420

HPLC Separation of Vitamin B

Primesep C Column Retention Control

Reverse Elution Order in Separation of Alkali Cation

Separation of Acetaminophen and 4-Aminophenol on Primesep C

Separation of Alginic Acid and Related Products

Separation of NyQuil Active Ingredients on Primesep C

Separation of Quaternary Amines

Separation Study of Fentin Acetate in Mixed-Mode HPLC

Separation Study of Zemuron Active Ingredient in Mixed-Mode HPLC

Primesep D is a reverse-phase analytical column with embedded basic ion-pairing groups specifically designed for direct plasma analysis.

Primesep B - Basic

Does not retain high-molecular weight plasma components
Retains hydrophobic and acidic components
All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 4)

Primesep D columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep D Application Examples

HPLC Method for Analysis of Levothyroxine Sodium Tablets

Analysis of Antibiotic Josamycin and Related Impurities in Mixed-Mode Chromatography

Analysis of Antibiotic Marbofloxacin and Related Impurities in Mixed-Mode Chromatography

Analysis of Antibiotic Norfloxacin and Related Impurities in Mixed-Mode Chromatography

Analysis of Lactic Acid on Primesep D Column

Analysis of Methylene Blue Purity in Reversed-Phase Cation-Exchange and Reversed-Phase Cation-Exclusion Modes

Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds

HPLC Analysis of Apple Extract using Mixed-Mode Chromatography

HPLC Analysis of Basic Drugs and Acidic Counter-Ions by Mixed-Mode Chromatography

HPLC Analysis of Bendamustine and Related Impurities

HPLC Analysis of Drugs in Saliva

HPLC Analysis of Gemfibrozil and Ibuprofen Using Mixed-Mode Chromatography

HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar

HPLC Analysis of Sulfate and Phosphate Ions on Primesep Columns

HPLC Application for Analysis of EDTA

HPLC Retention of Ethylglucuronide

HPLC Retention of Fentin Acetate

HPLC Retention of N-Cyclohexylsulfamic Acid

HPLC Retention of Polysorbate on Primesep D Column

HPLC Separation of 3-Nitrophthalic and 4-Nitrophthalic Acids on Primesep D HPLC Column

HPLC Separation of Aminobiphenyls

HPLC Separation of Antibiotics of Group in Mixed-Mode Chromatography

HPLC Separation of Benzalkonium Chloride BCT 8358 on Primesep D column

HPLC Separation of Benzenesulfonamide and Sulfanilamide

HPLC Separation of Benzenesulfonic and p-Toluenesulfonic Acids

HPLC Separation of Cetylpyridinium

HPLC Separation of Chloride and Nitrate Ions

HPLC Separation of Cidofovirs Mixture

HPLC Separation of Dequalinium and Benzonitrile on Different Columns

HPLC Separation of Dihydroxybenzoic Acid

HPLC Separation of Dyes

HPLC Separation of Hydrocortisone and Hydrocortisone’s Degradation Products

HPLC Separation of Organic Acids

HPLC Separation of Organics Acids

HPLC Separation of Phosphorous and Phosphoric Acids

HPLC Separation of Succinic and Methylmalonic Acids

HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC

HPLC Separation of Surfactants

HPLC Separation of Taurine

HPLC Separation of TRIS

HPLC Separation of Vanilmandelic, Hydroxyindoleacetic, and Homovanillic Acids

HPLC Separation of Vitamin C and Vitamin B2

Retention of Sialic Acid on Primesep D Column

Separation of Bisphenol A and Bisphenol A Glucoronide

Separation of Chlorate, Perchlorate, and Phosphonate Ions

Separation of Compounds in TCA Cycle on Primesep D Column

Separation of Phthalic Acids and Related Impurities

Separation Study of Fentin Acetate in Mixed-Mode HPLC

Primesep N is a normal-phase analytical column with embedded acidic groups.

Primesep N has embedded acidic groups with a pKa of about 5
Improves retention of basic compounds by cation-exchange mechanism at pH > 5
Separates polar compounds by HILIC mechanism
All mobile phases are LC-MS and preparative chromatography compatible

Primesep N columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 300 A, 100 A

Primesep N Application Examples

HPLC Separation Of Mixture of Pyridylacetic Acids

Effect of both pH and Organic Content on a Separation of Sugars, Amino

Acids, and Carboxylic Acids

HILIC Retention of Uridine and Uracil on Sielc’s HILIC Columns

HILIC Separation of Ritonavir and Ribavirin

HPLC Analysis of Pseudoephedrine and Citric Acid on Primesep Column

HPLC Derivatization and Quantitative Analysis of Glycerol

HPLC Method for Separation of Urea and Hydrogen Peroxide

HPLC Separation of Acetic acid and Acetic Hydrazide

HPLC Separation of Aromatic Diamine Disulfate

HPLC Separation of Citric Acid and Phosphate Ions in HILIC Chromatography

HPLC Separation of Heparines and Pentosan

HPLC Separation of Lecithin and Soy lecithin on Primesep N Column

HPLC Separation of Morpholino Sulfates

HPLC Separation of Organic Acids in HILIC Mode on Primesep N Column

HPLC Separation of Saccharin and Sorbitol

HPLC Separation of Sulfamic and Sulfuric Acid on Primesep N HILIC Column

HPLC Separation of Uridine and Uracil

Simultaneous Analysis of Glucose, Sodium, Potassium and Calcium Ions

Primesep P is a reverse-phase analytical column with embedded aromatic and acidic ion-pairing groups.

Primesep P - Phenyl

Improves retention of basic compounds by cation-exchange mechanism
Separates aromatic compounds by difference in pi-pi interaction
Retains neutral compounds by reverse-phase mechanism
All mobile phases are LC-MS and preparative chromatography compatible

Primesep P columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep P Application Examples
HPLC Separation of Aromatic Boronic Acids

Primesep SB is a reverse-phase analytical column with strong embedded basic ion-pairing groups.

It contains a C18 carbon chain and basic quaternary amine groups.

Primesep B - Basic

Improves retention of acidic compounds by anion-exchange mechanism
Separates bases by ion-exclusion mechanism
Retains neutral compounds by reverse-phase mechanism
All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 5)

Primesep SB columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 µm, 5 µm, 3 µm, 2.7 µm (solid core)
Pores: 100 A

Primesep SB Application Examples

HPLC Separation of Denatonium benzoate on Primesep SB Column

HPLC Determination of Vanillin on Primesep SB Column

HPLC Determination of Fosfomycin

HPLC Method for Analysis of Ascorbic acid and Sodium metabisulfite

HPLC Analysis of ACE Serum Active Ingredients

HPLC Analysis of Hop Extract (Beer Brewing)

HPLC Analysis of L-Cysteine-S-Sulfate on Primesep SB Column

HPLC Retention of EDTA in Sulfated CD

HPLC Retention of Ethylglucuronide

HPLC Retention of Sulfamic Acid on Primesep SB Mixed-Mode Column

HPLC Separation of 2-HEP and 2-HPP in the Presence of Methylcobalamin and Amino Acids

HPLC Separation of 5-Methyltetrahydrofolic Acid and Degradation Products

HPLC Separation of Alendronic, Ibandronic, Etidronic Acids

HPLC Separation of Ascorbic and Dehydroascorbic Acids

HPLC Separation of Epinephrine and Epinephrine Sulfonate

HPLC Separation of Methylphosphonic Acid and Related Products

HPLC Separation of Sodium, Chloride, Bromide, and Iodine

HPLC Separation of Sugar Phosphates

Separation of Nine Nucleotides by Mixed-Mode Chromatography

Separation of Nucleotide Monophosphates

Primesep 200 is a reverse-phase analytical column with embedded weak acidic ion-pairing groups

Improves retention of strong basic compounds by cation-exchange mechanism.

Retains neutral compounds by reverse-phase mechanism
Provides an additional interaction with polar molecules offering separation of isomers and structurally-related compounds
All mobile phases are LC-MS and preparative chromatography compatible

Reverse-phase analytical column Primesep 200

Primesep 200 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep 200 Application Examples

HPLC Determination of Perindopril

HPLC Method for Analysis of Metoprolol in Tablets

Separation of allo-D-isoleucine and L- Isoleucine

Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)

Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (1)

Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds

Generic Screening Method for Complex Mixtures

HILIC Separation of Hydroxypiperidine and Impurities

HPLC Analysis of Apple Extract using Mixed-Mode Chromatography

HPLC Analysis of Bendamustine and Related Impurities

HPLC Analysis of Citrulline and Arginine on Primesep Columns

HPLC Analysis of Piperazine on Primesep Columns

HPLC Analysis of the Catecholamine Pathway

HPLC Application for Determination of Melamine in Baby Formula

HPLC Application for Separation of Nucleotide Bases

HPLC Method for Analysis of Amphetamine

HPLC Method for Analysis of Azor

HPLC Method for Analysis of B-Estadiol

HPLC Method for Analysis of Bupivacaine

HPLC Method for Analysis of Bupropion

HPLC Method for Analysis of Butorphanol

HPLC Method for Analysis of Bystolic

HPLC Method for Analysis of Celebrex

HPLC Method for Analysis of Cetirizine

HPLC Method for Analysis of Cocaine

HPLC Method for Analysis of Crestor

HPLC Method for Analysis of Diclofenac

HPLC Method for Analysis of Etodolac

HPLC Method for Analysis of Fenofibrate

HPLC Method for Analysis of Fluconazole

HPLC Method for Analysis of Fluoxetine

HPLC Method for Analysis of Hydrochlorothiazide

HPLC Method for Analysis of Hydrocortisone

HPLC Method for Analysis of Hyoscyamine

HPLC Method for Analysis of Indapamide

HPLC Method for Analysis of Indomethacin

HPLC Method for Analysis of Ketoprofen

HPLC Method for Analysis of Lidocaine

HPLC Method for Analysis of Lipitor

HPLC Method for Analysis of Loratadine

HPLC Method for Analysis of MDA

HPLC Method for Analysis of MDMA

HPLC Method for Analysis of Meclizine

HPLC Method for Analysis of Methoxyphenamine

HPLC Method for Analysis of Montelukast

HPLC Method for Analysis of Nizatidine

HPLC Method for Analysis of Ofloxacin

HPLC Method for Analysis of Ondansetron

HPLC Method for Analysis of Paxil CR

HPLC Method for Analysis of Pindolol

HPLC Method for Analysis of Pioglitazone

HPLC Method for Analysis of Prednisolone

HPLC Method for Analysis of Principen

HPLC Method for Analysis of Procainamide Hydrochloride

HPLC Method for Analysis of Propranolol

HPLC Method for Analysis of Strychnine

HPLC Method for Analysis of Tamsulosin

HPLC Method for Analysis of Tetracaine

HPLC Method for Analysis of Tramadol

HPLC Method for Analysis of Trimipramine

HPLC Method for Analysis of Uloric

HPLC Method for Analysis of Valsartan

HPLC Method for Analysis of Verapamil

HPLC Method for Analysis of Vicodin

HPLC Retention of Aminopyrazole

HPLC Separation of 1-Methylhistidine and 3-Methylhistidine on Primesep 200

HPLC Separation of 8 Generic Compounds on Primesep Columns

HPLC Separation of Acidic, Basic and Zwitterionic Compounds on Special Columns for Retention of Polar Compounds

HPLC Separation of Acidic, Basic, and Neutral Compounds

HPLC Separation of Active Compounds in Drug Formulation

HPLC Separation of Agricultural Compounds Found in Tea

HPLC Separation of Amino Acids in Zero Organic Mode on Primesep 200 column

HPLC Separation of Caffeine and Phenylephrine

HPLC Separation of Catecholamines

HPLC Separation of Citrulline and Arginine

HPLC Separation of Codeine, Oxycodone, and Hydrocodone

HPLC Separation of Compounds of Catecholamine Pathway

HPLC Separation of Cotinine Metabolites

HPLC Separation of Creatine, Creatinine and Histidine

HPLC Separation of Creatine, Creatinine and Histidine with MS Conditions

HPLC Separation of DOPAC, Homovanillic Acid in Dopamine on the Primesep 200 Column

HPLC Separation of Epinephrine and Related Impurities

HPLC Separation of Fungicides in a Green Tea Matrix

HPLC Separation of Gentamicins

HPLC Separation of Guaifenesin and Terbutaline

HPLC Separation of Histamine and Phenethylamine

HPLC Separation of Neurotransmitters

HPLC Separation of Nucleobases (2)

HPLC Separation of Octopamine and Synephrine on Primesep 200 Column

HPLC Separation of Polar Compounds

HPLC Separation of Polyamines

HPLC Separation of Succinylcholine and Choline Using a Primesep 200 Column

HPLC Separation of Tetracyclines

HPLC Separation of Toluidine Isomers

Mixed-Mode HPLC Separation of Tertiary Amines on Primesep 200 Column

Neutral, Base, and Acid on Primesep 200 Column

Retention Characteristics of Betaine in Mixed-Mode HPLC

Separation of Deoxyribo Nucleic Acid

Separation of Diacid Hydrophobic and Ion Exchange Modes

Separation of Epinephrine Norepinephrine

Separation of Nucleic Bases

Separation of Simple Tertiary Amines

Separation of Sodium, Tetramethylammonium and Tetrabutylammonium on Primesep 200 Column

Separation of Toluidine and Aminobiphenyl Isomers

Primesep 500 is a reverse-phase analytical column with embedded acidic ion-pairing groups

Primesep 500 has an embedded carboxilic acid with a pKa of about 5
Improves retention of basic compounds by cation-exchange mechanism at pH > 5
Separates acids by ion-exclusion mechanism at a mobile phase pH > 5
Retains basic compounds, as any RP column, at pH < 5
Retains neutral compounds by reverse-phase mechanism
All mobile phases are LC-MS and preparative chromatography compatible

Primesep 500 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Primesep 500 Application Examples

HPLC Separation of Alkyl Sulfonic Acids

HPLC Separation of Glucosamine from Chondroitin Sulfate

Obelisc

Obelisc N is a column with very polar characteristics that works well for polar and charged analytes

Obelisc N offers both positively and negatively charged groups to interact with positively or negatively charged analytes
Multiple separation modes (NP, HILIC, IE)
In HILIC mode, Obelisc N offers different selectivity form other HILIC or bare silica columns
Mass spec, ELSD, preparative and low UV (<220 nm) compatible, low concentration buffers

Obelisc N columns are available in all standard dimensions

Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

Comparison of Obelisc N, SeQuant?® HILIC, and Bare Silica
Effect of both pH and Organic Content on a Separation of Sugars, Amino Acids, and Carboxylic Acids
Effect of Buffer on HPLC Separation of Buffers
HILIC Retention of 1,5-Naphthalenedisulfonic Acid on Obelisc N Mixed-Mode HILIC Columns
HILIC Separation of Aromatic Acids
HILIC Separation of Carboxylic Acids
HILIC Separation of Carboxymethylcysteine and Betaine
HILIC Separation of Common Preservatives – Citric Acid, Ascorbic Acid and EDTA
HILIC Separation of Epinephrine and Epinephrine Sulfonate
HILIC Separation of Polyethylene Glycol
HILIC Separation of Sugars on Obelisc N
HILIC Separation of Surfactant Triton X-100
HILIC Separation of Vitamins Group B
HPLC Analysis of TFA
HPLC Analysis of Trifluoroacetic and Acetic Acid on Obelisc N Mixed-Mode HPLC Column
HPLC Retention of Sulfuric Acid on Obelisc N Column
HPLC Separation of alpha-Aminobutyric, beta-Aminobutyric, and gamma-Aminobutyric acids on Obelisc N
HPLC Separation of Glyphosate Reaction Intermediates and Impurities
HPLC Separation of Nucleic Bases at pH 4 and 5 on Obelisc N
HPLC Separation of Polar and Hydrophobic Drugs on Obelisc R and N
HPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Obelisc N
HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities
Method for Analysis of Glyphosate in Apple Juice, Cranberry Juice, Mango Juice and Green Tea
Retention of Azide Ion on Obelisc N Column
Retention of N-Nitrosarcosine on Obelisc N Column
Retention of Taurine on Obelisc N Column
Retention of TFA on Atlantis and Obelisc N
Separation of Serotonin, Dopamine, and Related Compounds
Separation of Trisaccharides on SIELC Columns
Simultaneous Determination of Sodium and Sulfate Ions on Obelisc N Mixed-mode HILIC Column
Simultaneous Separation of Melamine and Cyanuric Acid

Obelisc R is a reverse-phase analytical column that can be used in traditional, reversed-phase type applications

Due to the presence of ionic groups and a long hydrophobic chain, Obelisc R offers additional retention and tuning that is not available with traditional reversed-phase columns
Multiple separation modes (RP, NP, IE)
Mass spec, ELSD, preparative and low UV (<220 nm) compatible, low concentration buffers

Obelisc R columns are available in all standard dimensions

Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A

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Primesep

Primesep 100 Application Examples

Obelisc

Arama